A Chemical Proteomics Approach to Reveal Direct Protein-Protein Interactions in Living Cells
نویسندگان
چکیده
منابع مشابه
A Chemical Proteomics Approach to Reveal Direct Protein-Protein Interactions in Living Cells.
Protein-protein interactions mediate essential cellular processes, however the detection of native interactions is challenging since they are often low affinity and context dependent. Here, we develop a chemical proteomics approach in vivo CLASPI [iCLASPI] (in vivo crosslinking-assisted and stable isotope labeling by amino acids in cell culture [SILAC]-based protein identification) relying upon...
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Post-translational modifications (PTMs) (e.g., acetylation, methylation, and phosphorylation) play crucial roles in regulating the diverse protein-protein interactions involved in essentially every cellular process. While significant progress has been made to detect PTMs, profiling protein-protein interactions mediated by these PTMs remains a challenge. Here, we report a method that combines a ...
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Post-translational modifications (PTM) of proteins can control complex and dynamic cellular processes via regulating interactions between key proteins. To understand these regulatory mechanisms, it is critical that we can profile the PTM-dependent protein-protein interactions. However, identifying these interactions can be very difficult using available approaches, as PTMs can be dynamic and of...
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The dynamics of protein-protein interactions play a central role in various biological processes including the function of multiprotein complexes and the regulation of signal transduction networks. Methods to sense or manipulate such interactions are therefore crucial for studying protein behavior in living cells. The interaction between two proteins can for example be enforced by the use of sm...
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Fluorescence imaging of fluorophore-labeled proteins opens a window into cellular protein biochemistry and enables direct visualization of protein dynamics, localization, and interactions in single living cells. Green fluorescent protein (GFP) and its color variants are popular fluorescent tags because they can be genetically fused to any protein of interest with great specificity. 2] However, ...
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ژورنال
عنوان ژورنال: Cell Chemical Biology
سال: 2018
ISSN: 2451-9456
DOI: 10.1016/j.chembiol.2017.10.001